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Image Search Results
Journal: Journal of Neurodevelopmental Disorders
Article Title: Effects of a ciliary neurotrophic factor (CNTF) small-molecule peptide mimetic in an in vitro and in vivo model of CDKL5 deficiency disorder
doi: 10.1186/s11689-024-09583-4
Figure Lengend Snippet: Effect of chronic oral P021 treatment on neuronal survival and dendritic spine maturation in the brain of Cdkl5 KO mice. A Number of BrdU-positive cells in the subgranular zone (SGZ) of the dentate gyrus (DG) of hippocampal sections from vehicle-treated (+ /Y n = 4, − /Y n = 3) and P021-treated (− /Y + P021 n = 4) Cdkl5 male mice. B Number of DCX-positive cells in the upper granular layer of the DG of hippocampal sections from vehicle-treated Cdkl 5 + /Y ( n = 3) and − /Y mice ( n = 3) and from P021-treated Cdkl 5 − /Y mice ( n = 4). C Examples of hippocampal sections processed for DCX immunostaining of one animal from each experimental group. D Number of NeuN-positive cells in the CA1 field of hippocampal sections from vehicle-treated Cdkl 5 + /Y and − /Y mice and from P021-treated Cdkl 5 − /Y mice as in B. E Examples of Golgi-stained hippocampal pyramidal neurons of one animal from each experimental group; red arrows are examples of mature mushroom-shaped spines. F Dendritic spine density in hippocampal pyramidal neurons of vehicle-treated (+ /Y n = 3, − /Y n = 3) and P021-treated (-/Y + P021 n = 3) Cdkl5 male mice. Data are expressed as number of spines per 10 µm. G Percentage of mature dendritic spines in relation to the total number of protrusions in hippocampal pyramidal neurons of Cdkl 5 + /Y and Cdkl 5 − /Y mice as in F. Values are represented as means ± SEM. ** p < 0.01, *** p < 0.001. Fisher’s LSD test after one-way ANOVA
Article Snippet: After drying, sections were rinsed with distilled water, stained in the developing solution of
Techniques: Immunostaining, Staining